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目的 :本研究拟通过观察电磁脉冲 (EMP)辐照大鼠后其海马C FOS表达的改变 ,初步探讨EMP致海马损伤的机制。方法 :用高场强电磁脉冲模拟源 (所产生的脉冲上升时间为 2 0ns ,脉宽为30 μs) ,在 2min内辐照大鼠 5次 ,并设对照组 ,辐照后于 1,6,12 ,2 4和 4 8h活杀动物 ,采用免疫组织化学SP方法显示C FOS的表达并在光镜 10× 4 0倍视野下 ,应用CMIAS Ⅱ图像分析仪对阳性细胞的图像学参数 积分光密度和平均光密度测定分析 ,最后结果经SPSS8.0统计软件统计分析。结果 :被辐照组大鼠在 6～ 12h时海马锥体细胞及齿状回颗粒细胞C FOS均呈高表达 ,积分光密度和平均光密度值在 12h时最大 ,且显著高于对照组 (P <0 .0 5和P <0 .0 1)。结论 :EMP可引起C FOS在海马锥体细胞及齿状回颗粒细胞的高表达 ,且C FOS可能在EMP致大鼠的脑损伤中起重要作用
OBJECTIVE: This study intends to investigate the mechanism of EMP-induced hippocampal damage by observing the changes of C FOS expression in the hippocampus of rats exposed to electromagnetic pulse (EMP). Methods: The high-field electromagnetic pulse simulation source (generated pulse rise time of 20ns, pulse width of 30 μs), 2 minutes irradiation in rats 5 times, and set the control group after irradiation at 1,6 , 12, 24 and 48 h live animals, immunohistochemical SP method was used to display the expression of C FOS and the light microscope 10 × 40 field of vision, using CMIAS Ⅱ image analyzer on the positive cells of the imaging parameters of the integrated light Density and average optical density determination analysis, the final results by SPSS8.0 statistical software statistical analysis. Results: The C FOS of hippocampal pyramidal cells and dentate gyrus granulosa cells in irradiated group were highly expressed at 6 ~ 12h, and the integral optical density and average optical density value were the highest at 12h and significantly higher than that of the control group P <0. 05 and P <0. 01). CONCLUSION: EMP can cause C FOS overexpression in hippocampal pyramidal cells and dentate gyrus granulosa cells, and C FOS may play an important role in EMP-induced brain injury in rats