论文部分内容阅读
【目的】研究湛江等鞭金藻(Isochrysis zhangjiangensis)由氮元素丰富至限制培养过程中脂质成分的变化。【方法】利用高效薄层色谱(HPTLC)分离分析微藻中脂质。【结果】随着培养基中营养物质的消耗,细胞逐渐处于胁迫状态,在这种状态下,细胞大量积累贮存脂质—甘油三脂(TAG),组成生物膜系统的单半乳糖甘油二脂(MGDG)、硫代异鼠李糖甘油二脂(SQDG)、磷脂酰甘油(PG)和磷脂酰胆碱(PC)含量降低,而游离脂肪酸(FFA)、甘油二脂(DAG)、双半乳糖甘油二脂(DGDG)、磷脂酰肌醇(PI)和磷脂酰乙醇胺(PE)则相对稳定。【结论】HPTLC可作为一种简便、可靠的微藻中脂质分离分析方法,为研究微藻油脂代谢途径以及甘油三脂(TAG)的调控积累提供有效手段。
【Objective】 The objective of this study was to investigate the changes of lipid components in Isochrysis zhangjiangensis from the enrichment of nitrogen to the limit of culture. 【Method】 The lipids in microalgae were separated and analyzed by high performance thin layer chromatography (HPTLC). 【Result】 With the depletion of nutrients in the medium, the cells gradually became in a state of stress. In this state, the cells accumulate and accumulate lipid-glycerol triacylglycerol (TAG) in large quantities, and mono galactosylglycerol MGDG, SQDG, PG, and PC were decreased, whereas free fatty acids (FFA), diglycerides (DAG) Lactose diglyceride (DGDG), phosphatidylinositol (PI) and phosphatidylethanolamine (PE) were relatively stable. 【Conclusion】 HPTLC can be used as a simple and reliable method for lipid separation and analysis in microalgae, providing an effective means for studying the lipid metabolism pathways and the regulatory accumulation of triglyceride (TAG) in microalgae.