镍对人是重要的致敏物质,又能使豚鼠致敏,但实验方法不同,致敏效果亦不同。作者用Hartley种白色豚鼠,对三种实验方法进行了比较:(1)GPM(Guinea pig maximiza-tion)法:本法诱导分二步,先将福氏完全佐剂(FCA)、5％硫酸镍水溶液及含5％硫酸镍的福氏完全佐剂分别注射到豚鼠脊侧皮内三点,各0.1 ml,两侧相同;一周后再用硫酸镍进行一次闭合斑贴试验(Finn Chambers)。激发用局部及全身两种方法:局部激发在致敏二周后用 Nickel is an important sensitizing agent to human beings, and can sensitize guinea pigs. However, different experimental methods result in different sensitization effects. The authors used Hartley white guinea pigs to compare three experimental methods: (1) GPM (Guinea pig maximiza-tion) method: This method was induced in two steps by first adding Freund’s complete adjuvant (FCA), 5% Nickel aqueous solution and Freund’s complete adjuvant containing 5% nickel sulfate were injected into the guinea pig’s spinal cord at three points, 0.1 ml each, and the two sides were the same; one week later, a patch test with nickel sulfate (Finn Chambers) was performed. Excitation with both local and systemic methods: local excitation in two weeks after the sensitization