论文部分内容阅读
目的考察大麻对正常小鼠脾细胞的免疫损伤作用。方法采用小鼠脾细胞体外培养法,在细胞培养液中加入100～200μg/mL大麻提取物,连续培养。检测小鼠脾细胞的ConA增殖反应、脾细胞凋亡现象和脾细胞培养上清中IL-2活性;观察脾细胞超微结构的改变和脾细胞内caspase-3活性。结果与正常对照组相比较,大麻染毒组小鼠脾细胞的ConA增殖反应和培养上清中IL-2的活性均显著降低,经Hoechst 33258染色可见大量凋亡的脾细胞;琼脂糖凝胶电泳显示为DNA梯形谱带;透射电镜观察发现100μg/mL大麻组小鼠脾细胞超微结构改变程度随接触时间延长而显著;荧光定量法检测显示100μg/mL大麻染毒组脾细胞内caspase-3活性显著升高。结论大麻提取物能引起小鼠脾淋巴细胞凋亡。
Objective To investigate the effect of marijuana on immune injury of normal mice spleen cells. Methods In vitro culture of mouse spleen cells was used. 100 ~ 200μg / mL cannabis extract was added into the cell culture medium and cultured continuously. The proliferation of splenocytes in mice was observed. The apoptosis of spleen cells and the activity of IL-2 in spleen cell culture supernatants were detected. The ultrastructural changes of splenocytes and the activity of caspase-3 in spleen cells were observed. Results Compared with the normal control group, the proliferation of splenocytes and the activity of IL-2 in the culture supernatant of the marijuana-treated mice were significantly decreased. A large number of apoptotic splenocytes were observed by Hoechst 33258 staining. The agarose gel Electrophoresis showed DNA ladder band; Transmission electron microscopy showed that 100μg / mL cannabinoid mice splenocytes ultrastructure changes with prolonged exposure time significantly; Fluorescent quantitative detection showed that 100μg / mL cannabis exposure group splenocyte caspase- 3 activity was significantly increased. Conclusion cannabis extract can cause apoptosis of splenic lymphocytes in mice.