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本文首次报道了瘤状多毛菌(Hirsutella nodulosa Petch)对二化螟越冬幼虫(Chilo suppressalis Walker)寄生性和瘤状多毛菌生物学特性的研究结果.其自然奇生致死率达73％,人工孢子接种致死率达93％;20℃、20天致死50％越冬幼虫的孢子量需8.2—10~6个/毫升;接种二化螟2—3代幼虫及其他6种不同代(包括越冬代)的鳞翅目幼虫均无寄生致死现象.瘤状多毛菌的最适发育温度为25～27℃、pH6～6.5;孢子萌发适宜温度为30℃左右、相对湿度70％以上:40℃以上孢子易失活,而菌体能存活15天左右.本文提出了一种适于该菌生长发育的培养基(蔗糖20、酵母汁10、KH_2PO_4 1.5、MgSO_4 0.5、CaCl_2·2H_2O 0.01、NaCl O.40克/升).
In this paper, we report for the first time the study of the biological characteristics of parasitism and tumorous Mycotoxins of Chilo suppressalis Walker by Hirsutella nodulosa Petch, which has a natural lethality of 73% and an artificial spore Inoculation lethality reached 93%; 20 ℃, 20 days dead 50% of overwintering larvae spores need to be 8.2-10 ~ 6 / ml; 2-3 generations of larvae inoculated with Chilo suppressalis and 6 other generations (including overwintering generation) Of lepidopteran larvae were not parasitic lethal phenomenon.The optimum temperature for the growth of tumorous Humicola was 25-27 ℃ and pH6-6.5.The suitable temperature for spore germination was about 30 ℃ and the relative humidity was above 70% Inactivation, and the bacteria can survive for about 15 days.In this paper, a suitable medium for the growth and development of the bacteria (sucrose 20, yeast juice 10, KH 2 PO 4 1.5, MgSO 4 0.5, CaCl 2 · 2H 2 O 0.01, NaCl O. 40 g /Rise).