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采用MTT法和流氏细胞仪技术 ,研究了天然药物鬼臼毒素 (podophyllotoxin)对人慢性髓细胞性白血病K5 6 2细胞凋亡及细胞周期的影响。结果表明 ,鬼臼毒素对K5 6 2细胞的IC50 是 79.1 9umol.L- 1 ;以IC50 药物浓度作用人K5 6 2细胞 2 4h ,细胞凋亡率达到 3 1 .96 % ,G0 -G1 期峰前有亚二倍体峰出现 ,此结果说明鬼臼毒素能快速有效地诱导K5 6 2细胞发生凋亡
The effect of podophyllotoxin, a natural drug, on the apoptosis and cell cycle of human chronic myeloid leukemia K562 cells was studied using MTT assay and Rhesi’s cytometry. The results showed that the IC50 of podophyllotoxin on K562 cells was 79.1 9umol.L-1; the effect of IC50 concentration on human K562 cells was 24h, the apoptosis rate reached 3 1.96%, G0-G1 peak The prediploid peak appears before, this result shows that podophyllotoxin can quickly and effectively induce the apoptosis of K562 cells.