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目的观察不同浓度P物质对体外培养的胃癌细胞的促增殖作用及其途径。方法人胃癌细胞系MKN45在RPMI 1640培养液中培养。采用免疫组织化学染色的方法观察胃癌细胞表达P物质特异性受体NK-1的情况。运用~3H-胸腺嘧啶脱氧核苷掺入指数作为判断细胞增殖的指标,向胃癌细胞的培养液中加入不同浓度的P物质,并运用SP的受体NK-1的特异性拮抗剂(ASN-1377642)后观察SP对胃癌细胞的增殖情况的改变。结果免疫组织化学染色方法证实,人胃癌细胞系MKN45表达SP的特异性受体NK-1;在一定的浓度范围内SP可促进低分化MKN45的增殖并呈剂量依赖性,受体NK-1的特异性拮抗剂可以抑制SP对胃癌细胞的促增殖作用。结论胃癌细胞表达SP的受体NK-1,SP对胃癌细胞有促增殖作用;其作用的完成是通过与受体NK-1结合有关。
Objective To observe the effects of substance P with different concentrations on the proliferation of gastric cancer cells cultured in vitro and its pathway. Methods Human gastric cancer cell line MKN45 was cultured in RPMI 1640 medium. Immunohistochemical staining was used to observe the expression of substance P-specific receptor NK-1 in gastric cancer cells. Using ~ 3H-thymidine incorporation index as an indicator of cell proliferation, different concentrations of substance P were added to the culture solution of gastric cancer cells and the specific antagonist of SP-1 receptor NK-1 (ASN- 1377642) observed the proliferation of gastric cancer cells SP changes. Results Immunohistochemical staining confirmed that human gastric cancer cell line MKN45 expressed SP-specific receptor NK-1. SP could promote the proliferation of low-differentiated MKN45 in a dose-dependent manner. Specific antagonists can inhibit SP on gastric cancer cells to promote proliferation. Conclusion The gastric cancer cells expressing SP receptors NK-1 and SP have the effect of promoting proliferation of gastric cancer cells. The completion of their actions is related to the binding of receptor NK-1.