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目的 通过流式细胞术 (FCM )检测外周血微核方法 ,研究氯化镉 (CdCl2 )染毒小鼠的外周血含微核网织红细胞率 ,评价流式细胞术在微核检测中的应用价值及探讨氯化镉的致突变作用。方法 采用转铁蛋白受体CD71荧光抗体和碘化丙啶 (PI)染色 ,并以疟原虫感染小鼠外周血红细胞为微核模式生物调校流式细胞仪 ,检测 10～ 6 0mg/kg环磷酰胺染毒和 5 0～ 4 0 0 μg/kg氯化镉染毒对小鼠的外周血含微核网织红细胞率的变化。 结果 不同剂量环磷酰胺染毒小鼠的外周血含微核网织红细胞率和骨髓含微核网织红细胞率均明显高于对照组小鼠 ,且二者之间相关性良好 (r =0 95 5 ) ;不同剂量氯化镉染毒小鼠外周血含微核网织红细胞率未见明显升高。结论 FCM测定外周血含微核网织红细胞率可替代传统显微镜检查骨髓含微核网织红细胞率用于遗传毒性评价 ;在本研究条件下未观察到氯化镉有明显的诱导小鼠外周血含微核网织红细胞形成的作用。
Objective To study the effect of flow cytometry (FCM) on micronucleated reticulocyte in peripheral blood of mice exposed to cadmium chloride (CdCl2) and to evaluate the application of flow cytometry in the detection of micronuclei Value and explore the mutagenic effect of cadmium chloride. Methods The transferrin receptor CD71 fluorescent antibody and propidium iodide (PI) staining were used. The peripheral blood red blood cells of mice infected with Plasmodium were detected by flow cytometry with micronucleus mode to detect 10-600 mg / kg The change of the rate of micronucleated reticulocyte in mice treated with phosphoramide and 50 ~ 400 μg cadmium chloride. Results The rates of peripheral blood micronucleated reticulocytes and bone marrow micronucleated reticulocytes in cyclophosphamide-treated mice were significantly higher than those in control mice (r = 0 95 5). There was no obvious increase of reticulocyte count in the peripheral blood of mice exposed to different doses of cadmium chloride. Conclusion FCM can be used to evaluate the genotoxicity of micronucleated reticulocyte in peripheral blood in order to replace the traditional microscopic examination of bone marrow containing micronucleated reticulocyte rate. No significant effect of cadmium chloride on the peripheral blood of mice was observed under the conditions of this study Containing micronucleated reticulocyte formation.