偏顶蛤胚胎和幼虫发育及温度对其浮游幼虫生长和发育的影响

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在室内条件下,人工诱导偏顶蛤(Modiolus modiolus)产卵并在显微镜下对其胚胎和浮游期幼虫的形态进行连续观察和拍照,并采用实验生态学的方法,研究温度对其浮游期幼虫生长和发育的影响。结果显示,偏顶蛤亲贝人工催产时,以流水刺激4 h,阴干12 h为宜。偏顶蛤卵呈圆形或卵圆形,沉性卵,卵径为(82.6±3.2)μm,精子鞭毛型,全长约50μm。在水温19~21℃下,偏顶蛤受精卵发育至D形幼虫(壳长104.0μm±4.3μm)、匍匐幼虫(壳长255.8μm±15.0μm)和早期稚贝(壳长329.1μm±5.8μm)分别历时20.5 h、20 d和36 d。水温是影响偏顶蛤幼虫生长和发育的主要因素之一,幼虫适宜水温为15~20℃,15℃组幼虫畸形率显著低于20℃组,生长率20℃组最高达5.4μm/d;水温25℃和30℃下,幼虫畸形率均显著高于15℃组和20℃组,并分别在第6天和第4天全部死亡。相对于高温而言,偏顶蛤幼虫对低温的耐受能力更强。本文旨在为偏顶蛤繁殖生物学和苗种繁育技术提供基础资料。 Under indoor conditions, Modiolus modiolus was induced to lay eggs and the morphological changes of embryos and planktonic larvae were observed and photographed under the microscope. Using experimental ecology, the effects of temperature on the larval stage larvae Effects of growth and development. The results showed that when the clam clam shellfish was artificially induced, it was advisable to stimulate it with flowing water for 4 hours and dry it for 12 hours. The clamshell eggs were round or oval, and were oviparous. The egg diameter was (82.6 ± 3.2) μm, and the sperm flagella were about 50 μm in length. At the water temperature of 19-21 ℃, the eggs of Dracaena coggygria developed to D shape larvae (shell length 104.0μm ± 4.3μm), creeping larvae (shell length 255.8μm ± 15.0μm) and early juveniles (shell length 329.1μm ± 5.8 μm) lasted 20.5 h, 20 d and 36 d, respectively. The water temperature is one of the main factors which affect the growth and development of the larvae. The suitable water temperature for larvae is 15-20 ℃, the deformity rate of larvae in 15 ℃ group is significantly lower than that in 20 ℃ group, and the growth rate is up to 5.4μm / d in 20 ℃ group. At 25 ℃ and 30 ℃, larvae deformity was significantly higher than those in 15 ℃ and 20 ℃ groups, all of which died on the 6th day and the 4th day respectively. Compared with the high temperature, the clam Larvae is more tolerant to low temperature. This article aims to provide the basic information for the clam breeding biology and seed breeding techniques.
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