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【目的】确定适宜丛枝菌根真菌与黄花蒿共生的土壤磷素水平,为黄花蒿优质高效生产提供理论依据。【方法】采用盆栽试验,检测已接种摩西球囊霉(Glomus mosseae)的黄花蒿在4种磷肥水平(0、40、80和120 mg/kg)下不同生育时期(早、中前期、中、中后和后期)的菌根侵染率、株高、茎基径等生长指标,以及叶绿素含量,收获后检测地上部干物重,枝和叶的氮、磷、钾含量,植株青蒿素含量。【结果】在各检测时期,施磷量80 mg/kg的处理菌根侵染率均最高,分别为59.7%、66.3%、76.7%、86.3%和89.0%;菌根化黄花蒿植株地上部干物重、株高、茎基粗和叶绿素含量在不同施磷处理间差异不显著(P>0.05);施磷可以促进菌根化黄花蒿植株对氮素的吸收,但施磷量超过120 mg/kg时促进氮素吸收的作用会下降。40 mg/kg以下的低磷处理有利于菌根化黄花蒿植株对磷的吸收。高磷和低磷条件下,菌根化黄花蒿对钾的吸收差异不显著(P>0.05),叶片钾含量为3.45%~3.95%。施磷量达到120 mg/kg时会显著降低菌根化黄花蒿的青蒿素含量(P<0.05)。【结论】40~80 mg/kg的施磷量最适宜丛枝菌根真菌与黄花蒿共生,植株有较高的青蒿素含量和生物产量。
【Objective】 The objective of this study was to determine the optimum level of soil phosphorus for symbiotic arbuscular mycorrhizal fungi and Artemisia annua and to provide a theoretical basis for high quality and efficient production of Artemisia annua. 【Method】 Pot experiments were conducted to investigate the effects of four kinds of phosphate fertilizer (0, 40, 80 and 120 mg / kg) on the growth of Artemisia annua L. inoculated with Glomus mosseae (early, middle, Midgut, and late), the growth index of plant height and stem diameter, and chlorophyll content. After harvest, the contents of nitrogen, phosphorus and potassium in shoots, branches and leaves were measured, and the content of plant artemisinin . 【Result】 The results showed that the mycorrhizal infection rates of the treatments with the phosphorus application rate of 80 mg / kg were the highest at 59.7%, 66.3%, 76.7%, 86.3% and 89.0%, respectively; There was no significant difference in dry matter weight, plant height, stem diameter and chlorophyll content between different treatments (P> 0.05). Phosphorus application promoted the uptake of nitrogen by Mycorrhiza plantation, / kg to promote the role of nitrogen absorption will decline. Phosphorus treatment below 40 mg / kg favored the uptake of phosphorus by mycorrhizal plants. Under high phosphorus and low phosphorus conditions, the absorption of potassium by mycorrhizal A. annua was not significant (P> 0.05), and the leaf potassium content was 3.45% -3.95%. The application of 120 mg / kg P could significantly reduce the artemisinin content of mycorrhizal A. annua (P <0.05). 【Conclusion】 The optimum phosphorus application rate for 40-80 mg / kg arbuscular mycorrhizal fungi symbiosis with Artemisia annua, plants have higher artemisinin content and biological yield.