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随着科研项目的广泛开展,要显示组织内的特殊结构,如脂肪组织或类脂等,需要采用冷冻切片。通常冷冻切片采用液体二氧化碳和氯乙烷等作制冷剂,目前也采用半导体致冷调节器来代替液体二氧化碳的冰冻。这些方法都要有一套特有的设备,并且操作起来也较繁复。我们采用液氮作制冷剂,对小鼠肝脏行冰冻切片,做脂肪染色,效果满意。做法: 1.取经给药后处死的小鼠肝脏,固定手中性甲醛液(内含90％生理盐水和10％福尔马林)中,2小时后将肝脏切开,以便充分固定,24小时后可行冰冻切片。 2.将一块湿滤纸平铺予标本头子(一般切片机均
With the extensive research projects, to show the special structure within the organization, such as adipose tissue or lipids, need to use frozen sections. Usually frozen sections using liquid carbon dioxide and ethyl chloride as a refrigerant, the semiconductor cooling regulator is also used to replace the liquid carbon dioxide frozen. These methods have a unique set of equipment, and operate more complicated. We use liquid nitrogen as a refrigerant, freezing frozen sections of the liver of mice to do fat staining, the results are satisfactory. Procedure: 1. Take the liver of the mice sacrificed after administration, fixed hands-free formaldehyde solution (containing 90% saline and 10% formalin), after 2 hours the liver is cut open, in order to fully fixed, 24 hours After the feasible frozen slice. 2. A piece of wet filter paper tiled to the standard head (usually slicer