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应用Ｂ淋巴细胞杂交瘤技术，以Ａ血型物质ＭＳＳ１０％２×（Ａ１）为抗原，经ＰＥＧ细胞融合，获得两株分泌ＩｇＡ类鼠抗人Ａ血型单抗杂交瘤细胞，细胞培养上清液效价为１０２４～２０４８，亲和力２～３ｓ。其中２Ｈ１０Ｅ１１杂交瘤细胞经血凝特异性试验、血凝抑制试验、吸收放散试验、红细胞标准谱细胞试验，表明２Ｈ１０Ｅ１１单抗仅对Ａ血型红细胞特异。２Ｈ１０Ｅ１１细胞置液氮中贮存３年，复苏传代后分泌抗体能力不变。单抗置５６℃水浴３０ｍｉｎ，－２０℃和３７℃反复冻融３次，效价不变。临床试用近百万人次，无一例错型，准确率１００％。证明可以替代标准血清，用于临床血型定型。
Using the technique of B lymphocyte hybridoma, two strains of IgA-like mouse anti-human A blood monoclonal antibody were obtained by fusion of PEG cells with MSS10% 2 × (A1) as the antigen, and the cell culture supernatant Price is 1024 ~ 2048, affinity 2 ~ 3s. Which 2H10 E11 hybridoma cells by hemagglutination specificity test, hemagglutination inhibition test, absorption test, erythrocyte standard cell line assay, indicating that 2H10 E11 mAb specific only to A blood group red blood cells. 2H10 E11 cells stored in nitrogen storage for 3 years, the ability to secrete antibodies after resuscitation passage unchanged. Monoclonal antibody was incubated in a water bath at 56 ° C for 30 min, frozen and thawed repeatedly at -20 ° C and 37 ° C for 3 times, and the titer remained unchanged. Nearly one million clinical trials, no case of error, accuracy rate of 100%. Proved to replace the standard serum for clinical blood typing.