目的：建立富集分离孕妇外周血胎儿有核红细胞的方法，并证实其为胎儿来源。方法：以血型糖蛋白Ａ（ＧＰＡ）为识别标志，对５例孕１２～４０周的孕妇外周血进行密度梯度离心富集胎儿有核红细胞，并经流式细胞分析及ＰＣＲ技术扩增Ｙ特异性序列以鉴定富集效果。结果：５例孕妇外周血富集后流式细胞分析结果，ＧＰＡ＋细胞含量平均为上层２３８％，下层１８１８％，富集后下层ＧＰＡ＋细胞含量是上层的７６倍；ＰＣＲ扩增结果，２例阳性，３例阴性，所得结果与实际性别吻合。结论：胎儿有核红细胞的富集及鉴定为无损伤性产前诊断的临床应用开辟了诱人前景。 Objective: To establish a method to enrich fetal nucleated erythrocytes isolated from peripheral blood of pregnant women and confirm its origin as fetus. Methods: Peripheral blood mononuclear cells (PBMCs) from 5 pregnant women with gestational age of 12 to 40 weeks were collected by density gradient centrifugation with glycoprotein A (GPA) as a marker. Fetal nucleated erythrocytes were enriched by flow cytometry and PCR Sex sequence to identify enrichment effect. Results: The peripheral blood of 5 pregnant women were collected by flow cytometry. GPA + cells averaged 238% of the upper layer and 1818% of the lower layer, while the lower layer of GPA + cells were 76 times of the upper layer after enrichment. Increasing results, 2 cases were positive, 3 cases were negative, the results obtained and the actual sex. CONCLUSION: Enrichment and identification of fetal erythrocytes opens up attractive prospects for the clinical use of non-invasive prenatal diagnosis.