目的 研究不同采样方法对手部卫生学评价的影响, 以便寻找最佳采样方法.方法 采用3种不同采样方法, 对手部细菌采集准确性进行比较和评价.结果 手印法采集手上细菌捕获菌数最低, 其次是棉拭子涂抹法采集捕获菌量也比较低且可重复性较差;以五指擦洗法采集的手部细菌总数最高, 采样结果比较稳定, 结果重复性好且操作方便.结论 五指擦洗法是进行手消毒效果评价时对手部细菌较科学和客观的采样方法.“,”Objective To study the influence of different sampling methods on hand hygiene evaluation, so as to find a best sampling method. Methods The accuracy of hand bacteria sampling was compared and evaluated by three different sampling methods. Results The amount of bacteria captured by handprint method was the lowest, then the amount of bacteria captured by the cotton swab smearing method was low too and the repeatability was poor. The amount of hand bacteria collected by the five-finger scrubbing method was the highest, and the results were stable, reproducible and easy to operate.Conclusion Five-finger scrubbing method was a scientific and objective sampling method of the hand bacteria when evaluating the effect of hand disinfection.