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目的研究病理和正常生理状态下淫藤骨痹康方总萜体内代谢指纹图谱差异,阐明其活血作用的药效物质基础。方法采用HPLC法建立藤骨痹康方总萜在正常生理状态与急性血瘀病理状态下的代谢特征指纹图谱,并对共有峰的数与量进行对比分析。结果在正常生理状态下,5个批次的含药血浆样品中有21个共有峰,其中3个峰来自于空白血浆,4个峰为总萜的原型成分,14个峰为其代谢产物峰或产生的新成分峰;在急性血瘀病理状态下,含药血浆样品中有21个共有峰,其中6个峰来自于空白血浆,3个峰为总萜的原型成分,12个峰为其代谢产物峰或产生的新成分峰;经对照品对照,52min处的峰为丹参酮IIA。总萜在正常生理状态与病理状态下的色谱峰的数目、峰形及峰的大小存在一定的差异;病理状态下的色谱峰峰面积明显强于正常生理状态下的色谱峰峰面积,其中有7个共有峰的峰面积具有显著性差异(P<0.05)。结论淫藤骨痹康方总萜体内代谢指纹图谱的建立方法可行;不同生理状态下的大多数共有峰的峰面积存在显著性差异;其入血原型成分与代谢新成分可能为其活血作用的药效物质。
Objective To study the difference of the metabolic fingerprints of total triterpene in JING Teng Gu Bi Bi Kang Fang under pathological and normal physiological conditions and elucidate the pharmacodynamic basis of its role in activating blood circulation. Methods HPLC method was used to establish the metabolic fingerprint of the total terpenoids of Tengu Bi Bi Kang Fang in the normal physiological state and the pathological state of acute blood stasis. The number and quantity of the common peaks were compared and analyzed. Results Under normal physiological conditions, there were 21 common peaks in 5 batches of drug-containing plasma samples, of which 3 were from blank plasma, 4 were the prototype components of total terpenoids and 14 were their metabolite peaks Or generated new peak; in the pathological condition of acute blood stasis, there are 21 common peaks in the drug-containing plasma samples, of which 6 peaks come from the blank plasma, 3 peaks are the prototype components of total terpenes, and 12 peaks are Metabolite peaks or new peak components produced; the reference substance control, the peak at 52min tanshinone IIA. Total terpene in the normal physiological state and pathological state of the number of peaks, peak shape and the size of the peak there is a certain difference; pathological state of the chromatographic peak area was significantly stronger than normal physiological peak chromatographic peak area, of which The peak area of seven common peaks had significant difference (P <0.05). Conclusion The method of establishing metabolic fingerprinting of the total terpene in JING TANGGU BIKANG is feasible. The peak areas of most common peaks in different physiological states have significant difference. The prototypical components and new components of metabolism may be their activating blood Effective substances.