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[目的]探讨乙型肝炎病毒(HBV)前S1抗原(PreS1Ag)与HBV-DNA和HBeAg之间的关系,以评价其作为判定饮服人群传播HBV危险性指标时的作用,为修订相应管理法规提供依据。[方法]用ELISA法对450例HBsAg阳性标本进行PreS1Ag和HBV血清标志物的检测,用实时荧光定量PCR测定HBV-DNA。[结果]PreS1Ag在HBV-DNA和HBsAg阳性血清中的检出率均明显高于相应的阴性组(均为P﹤0.01)。HBeAg阴性组中PreS1Ag的检出率为43.0%,PreS1Ag与HBV-DNA的符合率为70.6%,与HBeAg的符合率为68.45%。[结论]PreS1Ag与HBV-DNA、HBeAg呈良好的相关性。作为反映HBV感染和复制的指标,PreS1Ag较HBeAg更敏感。无条件检测HBV-DNA而HBeAg阴性的血清,检测前S1抗原,对控制传染源更有意义。
[Objective] To investigate the relationship between PreS1Ag and HBV-DNA and HBeAg in hepatitis B virus (HBV) to evaluate the role of preS1Ag in determining the risk of HBV transmission in the diet service population. In order to revise the corresponding management regulations Provide evidence. [Method] 450 samples of HBsAg positive samples were detected by ELISA for PreS1Ag and HBV serum markers, and HBV-DNA was detected by real-time fluorescence quantitative PCR. [Results] The detection rates of PreS1Ag in HBV-DNA and HBsAg-positive sera were significantly higher than those in the corresponding negative group (all P <0.01). The detection rate of PreS1Ag in HBeAg-negative group was 43.0%, the coincidence rate of PreS1Ag and HBV-DNA was 70.6%, and the coincidence rate with HBeAg was 68.45%. [Conclusion] PreS1Ag has a good correlation with HBV-DNA and HBeAg. As an indicator of HBV infection and replication, PreS1Ag is more sensitive than HBeAg. Unconditional detection of HBV-DNA and HBeAg-negative serum, detection of pre-S1 antigen, more meaningful to control the source of infection.