Objective:To determine the chemical composition of essential oils and lipid constituents of Ballota andreuzziana (B. andreuzziana), Teucrium zanonii (T. zanonii)andVerbena tenuisecta (V. tenuisecta) growing in Libya, and to test the antibacterial activity of different extracts ofTeucrium zanonii.Methods: The volatile oils of all plants were extracted by hydrodistillation method and analyzed byGC/MS method. The lipid constituents of plants were obtained by extraction with petroleum ether and fractionated into fatty alcohols, fatty acids and unsapoinfiable matters. Antibacterial activity ofT. zanonii extracts and antioxidant activity of different extracts ofT. zanonii were also studied.Results: The volate oil ofB. andreuzziana was found to consists of20 compounds in which caryophyllin is the main one (63.1%), the volatile oil ofT. zanonii consists of74 compounds in which germacrene-D was the main compound, while the volatile oil ofV. tenuisecta consists of13compounds with1-octen-3- ol as a major constituent(52.87%). The study of antimicrobial activity of different extracts ofV. tenuisecta showed that, both methanol and butanol extracts exhibited the highest activity againstMycobactirium phlei (M. phlei) andCandida albicans (C. albicans) respectively, while petroleum ether, fatty alcohols and unsaponifiable fractions had no antimicrobial activity against all the tested microorganisms. Investigation of the antioxidant activity of different extracts ofT. zanonii usingDPPH method proved that, the ethyl acetate and butanol fractions showed the highest activity where the inhibition percentage(I%) are 93.6 and92.1 respectively.Conclusions:This is the first report about the volatile oils of these plants whereT. zanonii have the highest content and the highest number of the identified compounds. The study of antioxidantT. zanonii extracts proved that, the ethyl acetate, butanol and aqueous extracts have the highest antioxidant activity. Methanol and butanol extracts ofV. tenuisecta exhibited the highest activity againstM. phlei andC. albicans respectively.