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目的 探讨胃镜直视下刷取胃粘膜脱落细胞中端粒酶活性对胃癌的诊断价值。方法 采用TRAP PCR ELISA法对 5 6例胃组织和胃粘膜刷落细胞进行端粒酶活性的分析。结果 胃癌组端粒酶活性水平 (平均A值 0 .745 )明显高于胃炎组 (平均A值 0 .0 6 8)。端粒酶活性水平在胃刷落细胞中与组织的是相近的。端粒酶在胃癌组的阳性率为90 % ,明显高于胃刷落细胞涂片的阳性率 (6 6 .7% )、血CEA的阳性率 (5 6 .7% )。同时还测得 75 %的胃粘膜不典型增生者端粒酶活性水平也显著升高。结论 胃镜直视下刷取胃粘膜脱落细胞中端粒酶活性测定可作为诊断胃癌有效而敏感的方法。
Objective To investigate the diagnostic value of telomerase activity in exfoliative cells of gastric mucosa under gastroscope for gastric cancer. Methods The telomerase activity was analyzed in 56 gastric tissue and gastric mucosal brushing cells using TRAP PCR ELISA. Results The telomerase activity level in gastric cancer group (average A value of 0.754) was significantly higher than that in gastritis group (average A value of 0.66). The level of telomerase activity is similar to that of tissue in gastric brushing cells. The positive rate of telomerase in gastric cancer group was 90%, which was significantly higher than the positive rate of gastric brushing cell smear (66.7%) and the positive rate of blood CEA (56.7%). At the same time, telomerase activity was also significantly increased in 75% of gastric dysplasia patients. Conclusion Endoscopic examination of telomerase activity in exfoliative cells of gastric mucosa under gastroscope can be used as an effective and sensitive method for the diagnosis of gastric cancer.