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目的研究高血压病患者过氧化物酶体增殖物激活受体γ2基因(PPARγ2)Pro12Ala多态性与认知功能之间的关系。方法纳入2008年1月至2009年2月在四川大学华西医院心内科就诊的四川地区汉族人群中原发性高血压患者502例,收集一般资料,采用国际通用的简易智力状况量表(MMSE)测验认知功能,按认知评分分为认知功能正常组(n=376)和认知功能障碍组(n=126),用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术测定PPARγ2基因Pro12Ala多态性。同时测定胆固醇、甘油三酯、空腹血糖、胰岛素及餐后2 h血糖、胰岛素水平。结果 502例原发性高血压患者等位基因型及基因频率的分布结果显示,PP基因型88.6%,PA基因型11.4%,AA基因型0%,P等位基因频率94.3%,A等位基因频率5.7%。其中认知功能正常组376例,PP和PA基因型分别为328例(87.2%)和48例(12.8%);认知功能障碍组126例,PP和PA基因型分别为126例(92.9%)和9例(7.1%)。两组基因型及等位基因频率分布显示差异均无统计学意义(P均>0.05),进一步按照性别和年龄分组后,认知功能正常组与认知功能障碍组之间基因型间差异无统计学意义。Logistic回归分析也未发现PPARγ2基因Pro12Ala多态性与高血压病认知功能相关〔PP基因型:OR=1;PA基因型:OR=0.511,95%可信区间(0.226,1.153),P=0.106〕。结论 Pro12Ala基因多态性与高血压病患者认知功能障碍无明显相关性。
Objective To investigate the relationship between the polymorphisms of peroxisome proliferator activated receptor gamma 2 gene (PPARγ2) Pro12Ala and cognitive function in patients with essential hypertension. Methods From January 2008 to February 2009, 502 cases of essential hypertension in Sichuan Han population from Sichuan University, Department of Cardiology, West China Hospital of Sichuan University were enrolled in this study. General information was collected and the MMSE was used. The cognitive function was assessed according to the cognitive score and divided into normal cognitive function group (n = 376) and cognitive dysfunction group (n = 126), and polymerase chain reaction-restriction fragment length polymorphism ) Technique was used to detect the PPARγ2 gene Pro12Ala polymorphism. Simultaneous determination of cholesterol, triglycerides, fasting blood glucose, insulin and 2 h postprandial blood glucose, insulin levels. Results Distribution of allele and gene frequencies in 502 patients with essential hypertension showed that 88.6% of PP genotypes, 11.4% of PA genotypes, 0% of AA genotypes, 94.3% of P allele frequencies and A allele Gene frequency 5.7%. There were 376 cases (87.2%) and 48 cases (12.8%) of PP and PA genotypes in normal cognitive function group, 126 cases in cognitive dysfunction group, and 126 cases (92.9% ) And 9 cases (7.1%). There was no significant difference in genotype distribution and allele frequencies between the two groups (P> 0.05). After grouped according to gender and age, there was no significant difference in genotype between normal cognitive function group and cognitive dysfunction group Statistical significance. Logistic regression analysis also did not find PPARγ2 gene Pro12Ala polymorphism associated with cognitive function of hypertension (PP genotype: OR = 1; PA genotype: OR = 0.511, 95% confidence interval (0.226,1.153), P = 0.106]. Conclusion There is no significant correlation between Pro12Ala gene polymorphism and cognitive dysfunction in patients with hypertension.