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目的采用不同浓度丹参酮IIA磺酸钠(STS)治疗火器伤引起豚鼠ALI时的肺保护作用。方法60只豚鼠随机分为正常对照组为N组、模型组为M组、生理盐水对照组为P组、低剂量STS治疗组(A组)、中剂量STS治疗组(B组)、高剂量STS治疗组(C组),每组10只,制作豚鼠ALI动物模型。造模0.5h后,采用免疫印迹法测定细胞核因子(NF-κB),用光学显微镜观察实验豚鼠病理变化情况,并及时记录其观察结果。结果病理学结果除N组外,造模12h各组动物的肺泡结构破坏,结构混乱,各种炎性细胞浸润,以M组最明显;用丹参酮IIA干预治疗各组中均有改善,以中等浓度STS干预治疗为最有效(P<0.05)。结论STS治疗对火器伤引起的豚鼠ALI具有保护作用,以STS中剂量保护肺作用最明显。
Objective To observe the protective effects of sodium tanshinone IIA sulfonate (STS) on guinea pig ALI induced by firearm injuries. Methods Sixty guinea pigs were randomly divided into normal control group (N group), model group (M group), saline control group (P group), low dose STS treatment group (A group), middle dose STS treatment group (B group) STS treatment group (C group), each group of 10, making guinea pig ALI animal model. After modeling for 0.5h, the nuclear factor (NF-κB) was determined by immunoblotting. The pathological changes of experimental guinea pigs were observed with optical microscope and the results were recorded timely. Results The results of pathology showed that the alveolar structure was disrupted, the structure was disorganized and all kinds of inflammatory cells were infiltrated in all groups except the N group, the most obvious was in the M group. The improvement was observed in the groups treated with the tanshinone IIA Concentration of STS intervention was the most effective (P <0.05). Conclusion STS treatment has a protective effect on guinea pig ALI induced by firearm injury and the most obvious effect is to protect lung with STS medium dose.