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经连续4次大鼠尾静脉注射免抗大鼠thy－1抗血清（ATS），建立大鼠thy－1系膜增生性肾小球肾炎模型。与此同时注射肝素，通过肾小球细胞计数、增殖细胞核抗原（PCNA）阳性细胞数及a一平滑肌肌动蛋白（a－SMA）免疫酶标染色，以观察肝素对肾小球系膜细胞增生的影响；用过碘酸六胺银（PASM）和Ⅳ型胶原（ColⅣ）免疫酶标染色观察肝素对肾小球系膜基质的作用。结果发现肝素组与正常组之间，上述指标均受到明显的抑制，提示肝素对大鼠thy—1至肾炎有良好的治疗效果。
Rat thy-1 mesangial proliferative glomerulonephritis model was established by injecting anti-rat thy-1 antiserum (ATS) into tail vein of rats for 4 consecutive times. At the same time, heparin was injected, and the number of glomerular cells, the number of proliferating cell nuclear antigen (PCNA) positive cells and the alpha-smooth muscle actin (a-SMA) immunoenzyme staining were used to observe the effects of heparin on glomerular mesangial cell proliferation . The effect of heparin on glomerular mesangial matrix was observed by immunoenzyme staining with PASM and ColⅣ. The results showed that heparin group and the normal group, the above indicators were significantly inhibited, suggesting that heparin on rats thy-1 to nephritis have a good therapeutic effect.