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以IR36(indica)和热研2号(japonica,广亲和品种)为亲本,构建了包含180个单株的F2群体及包括110个标记的分子连锁图谱。利用该F2群体,进行了水稻花粉不育数量性状基因座(quantitative trait locus,QTL)的检测和遗传效应分析,共检测到3个花粉不育QTL,分别位于第3、57、染色体上,此外,共检测到9个由雄配子引起的偏分离QTL,其中7个与ga-14和ga-11位点的配子败育类型相同。与花粉形态鉴定相比,偏分离的数据对检测F1杂种花粉败育基因更为敏感。在第56、染色体上控制偏分离的2个QTL位点,其杂合基因型出现的频率偏高。在qHPS-5位点,粳型纯合子表现出比杂合子和籼型纯合子更低的育性水平。本研究获得的分子标记将有助于聚合尽可能多的中性亲和基因以解决亚种间F1杂种的花粉不育性问题。
The F1 population containing 180 individuals and the molecular linkage map including 110 markers were constructed using IR36 (indica) and japonica (broad compatibility variety) as parents. In this F2 population, the quantitative trait locus (QTL) detection and genetic effect analysis of rice pollen sterility were carried out. Three pollen sterility QTLs were detected on chromosomes 3,57 and chromosomes, respectively A total of nine QTLs for segregation induced by male gametes were detected, of which seven were of the same type as the gametes of ga-14 and ga-11. Partial segregation data is more sensitive to F1 pollen abortion than pollen morphological identification. At locus 56, two heterozygous QTL loci on the chromosome control their heterozygous genotypes at higher frequencies. At the qHPS-5 locus, japonic homozygotes show lower fertility levels than heterozygous and indica homozygotes. The molecular markers obtained in this study will help to aggregate as many neutral affinity genes as possible to solve the pollen sterility problem of intersubspecific F1 hybrids.