[Objective]To research the effects of preculture on the cryopreservation by encapsulation-vitrification of Dioscorea opposita Thunb.cv. Tiegun. [Methods] Tissue culture method was adopted; all the preculture tests were carried out under 4 ℃. [Results]Sucrose concentration,penetrant types,preculture time and preculture method had significant impacts on the relative survival rate and regeneration rate of D.opposita nodes after cryopreservation by encapsulation-vitrification. The appropriate sucrose concentration and precultured time were 0. 2 mol /L and 3 d,respectively. When 0. 1 mol / L sucrose + 0. 1 mol / L glycerol was taken as the penetrant,the relative survival rate and regeneration rate were the maximum after cryopreservation by encapsulation-vitrification. Effect of direct preculture for 3 d on MS + 0. 1 mol / L sucrose +0. 1 mol / L glycerol was the best,which could obtain the highest relative survival rate( 89%) and regeneration rate( 52%) after cryopreservation. Therefore,plantlet nodes of D. opp. Thunb. cv. Tiegun should be precurtured on MS + 0. 1 mol / L sucrose + 0. 1 mol / L glycerol for3 d at 4 ℃. [Conclusions] This research provides a technology system for vitrification,and the method is simple and effective. [Objective] To research the effects of preculture on the cryopreservation by encapsulation-vitrification of Dioscorea opposita Thunb.cv. Tiegun. [Methods] Tissue culture method was adopted; all the preculture tests were carried out at 4 ° C. [Results] Sucrose concentration , penetrant types, preculture time and preculture method had significant impacts on the relative survival rate and regeneration rate of D.opposita nodes after cryopreservation by encapsulation-vitrification. The appropriate sucrose concentration and precultured time were 0. 2 mol / L and 3 d, respectively. When 0.1 mol / L sucrose + 0.1 mol / L glycerol was taken as the penetrant, the relative survival rate and regeneration rate were the maximum after cryopreservation by encapsulation-vitrification. Effect of direct preculture for 3 d on MS +0.1 mol / L sucrose +0. 1 mol / L glycerol was the best, which could obtain the highest relative survival rate (89%) and regeneration rate (52%) after cryopreservation. Therefore, p Lantlet nodes of D. opp. Thunb. cv. Tiegun should be precurtured on MS + 0.1 mol / L sucrose + 0.1 mol / L glycerol for 3 d at 4 ° C. [Conclusions] This research provides a technology system for vitrification , and the method is simple and effective.