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目的探讨嗜肺军团菌感染Balb/c小鼠后血清白介素12(IL-12)水平和巨噬细胞功能的变化。方法将36只健康Balb/c小鼠随机分为观察组和对照组各18例,观察组小鼠经腹腔注射嗜肺军团菌菌液0.10 m L,对照组小鼠则经腹腔注射等量的生理盐水,分别于注射后1周和1个月,检测并比较两组小鼠的血清IL-12水平和巨噬细胞功能。结果注射1周后,观察组小鼠的血清IL-12水平为(14.98±2.90)Lg/m L,巨噬细胞的杀伤活性为(34.71±7.28)%,较对照组均有明显降低,组间比较差异均有统计学意义(P<0.05);注射1个月后,观察组小鼠的血清IL-12水平及巨噬细胞杀伤活性均有显著回升,组间比较异均无统计学意义(P>0.05)。结论嗜肺军团菌感染Balb/c小鼠后1周,小鼠的血清白介素12水平及巨噬细胞的功能均有显著下降,且于注射后1个月恢复正常,临床上可将感染后1个月作为相关血清抗体的最佳制备时间。
Objective To investigate the changes of serum interleukin 12 (IL-12) and macrophage function in Legionella pneumophila infected Balb / c mice. Methods Thirty-six healthy Balb / c mice were randomly divided into observation group (18 cases) and control group (18 cases). The mice in the observation group were intraperitoneally injected with Legionella pneumophila bacteria solution (0.10 mL), while the control group Normal saline, respectively, at 1 week and 1 month after injection, serum IL-12 level and macrophage function in two groups were detected and compared. Results After 1 week of injection, the level of IL-12 in the observation group was (14.98 ± 2.90) Lg / m L and that of macrophages was (34.71 ± 7.28)%, which was significantly lower than that in the control group (P <0.05). After 1 month of injection, serum IL-12 level and macrophage killing activity of the mice in the observation group all increased significantly, with no significant difference between the two groups (P> 0.05). Conclusion One week after Legionella pneumophila infection Balb / c mice, serum interleukin-12 level and macrophage function in mice were significantly decreased, and returned to normal one month after injection. Clinically, Months as the best preparation time of the relevant serum antibody.