用过氧化氢氧化还原型谷胱甘肽制备了氧化型谷胱甘肽,并优化反应条件为:还原型谷胱甘肽浓度200～300 g/L,反应pH 5.0～6.0,过氧化氢终浓度3%。可得纯度99.5%的氧化型谷胱甘肽,总收率为95.9%。考察了产物对对乙酰氨基酚诱导的小鼠急性肝损伤的治疗作用,结果显示能显著降低谷丙转氨酶和谷草转氨酶的活性,且与还原型谷胱甘肽没有显著性差异。 Oxidized glutathione was prepared by hydrogen peroxide reduction of glutathione, and the optimal reaction conditions were as follows: the concentration of reduced glutathione was 200-300 g / L, the reaction pH was 5.0-6.0, and the final hydrogen peroxide Concentration of 3%. An oxidized glutathione with a purity of 99.5% was obtained with an overall yield of 95.9%. The therapeutic effect of the product on acetaminophen-induced acute liver injury in mice was investigated. The results showed that the activity of alanine aminotransferase and aspartate aminotransferase was significantly decreased, and there was no significant difference between the product and reduced glutathione.