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目的探讨异丙酚对Wistar大鼠幼鼠癫痫持续状态的控制疗效及脑保护作用。方法将60只Wistar大鼠随机分为4组,空白对照组,致痫组(异丙酚组,安定组,盐水组)。戊四氮(PTZ)诱导大鼠SE,建立SE模型。分别给予腹腔注射止抽药物,记录各组控制SE的时间(min)。并于止抽后24h、72h、7d断头,取脑,常规病理切片,切取海马组织,染色,光镜及电镜下观察海马结构形态学改变。应用流式细胞术和琼脂糖凝胶电泳,检测海马神经元细胞凋亡,证实异丙酚对脑保护作用和脑神经元细胞凋亡的影响。结果异丙酚组控制癫痫持续时间与地西泮组无显著差异(P>0.05)。异丙酚组神经元细胞大致正常,仅少数出现线粒体肿,细胞结构出现轻微改变。较其他组改变轻微。流式细胞术和琼脂糖凝胶电泳检测可见,异丙酚组神经元细胞凋亡数量较其他组少。结论异丙酚可以有效控制癫痫持续状态。形态学观察说明,异丙酚对脑细胞有一定的保护作用。流式细胞术检测和琼脂糖凝胶电泳实验证实,异丙酚则对神经元凋亡有有效的抑制作用,可以减少神经元的凋亡,保护脑细胞。异丙酚用于癫痫持续状态的治疗,并对SE脑损伤的远期后果有其有效的防治价值。
Objective To investigate the effect of propofol on the status of epilepsy in young rats with Wistar rats and the protective effect of propofol on it. Methods Sixty Wistar rats were randomly divided into 4 groups: blank control group, epileptogenic group (propofol group, diazepam group and saline group). Rats were induced by pentylenetetrazole (PTZ) to establish SE model. Respectively intraperitoneal injection of anti-smoking drugs, recording the time of each group control SE (min). The rats were sacrificed 24 h, 72 h, 7 d after stop pumping, and the brain and routine pathological sections were taken. The hippocampus was cut and stained. The morphological changes of hippocampus were observed under light microscope and electron microscope. Flow cytometry and agarose gel electrophoresis were used to detect neuronal apoptosis in hippocampus and to verify the effect of propofol on neuroprotection and neuronal apoptosis. Results There was no significant difference in the duration of epilepsy between propofol group and diazepam group (P> 0.05). Propofol group, normal neurons cells, only a few appear mitochondrial swelling, cell structure showed a slight change. Change slightly than other groups. Flow cytometry and agarose gel electrophoresis showed that propofol group neurons apoptosis less than other groups. Conclusion Propofol can effectively control the status of epilepsy. Morphological observation shows that propofol has a certain protective effect on brain cells. Flow cytometry and agarose gel electrophoresis experiments confirmed that propofol can effectively inhibit neuronal apoptosis, can reduce neuronal apoptosis and protect brain cells. Propofol is used in the treatment of status epilepticus, and its long-term consequences of brain injury have its effective prevention and treatment value.