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目的克隆金铁锁糖基转移酶全长基因,并进行生物信息学分析。方法根据转录组数据,反转录获得全长c DNA,通过生物信息学软件对该基因蛋白特性及系统发育树进行分析。结果克隆得到c DNA全长序列,命名为UGT71G1,该c DNA全序列长1402 bp,包含一条1107 bp完整开放阅读框,编码368个氨基酸,预测相对分子质量为41.05KD,等电点6.03,在155位到336位置处存在高度保守UDPGT结构功能域,经多重序列比对与同科植物香石竹具有很高的同源性。结论成功克隆、分析了金铁锁UGT71G1基因,为金铁锁有效成分合成关键酶基因及调控机制研究提供基础。
Objective To clone the full-length gene of Ginkgo aculeatum glycosyltransferase and analyze its bioinformatics. Methods According to the transcriptome data, full length c DNA was obtained by reverse transcription, and the characteristics of the protein and phylogenetic tree were analyzed by bioinformatics software. Results The full length cDNA of c DNA was cloned and named as UGT71G1. The full length cDNA of c DNA was 1402 bp in length and contained a 1107 bp complete open reading frame encoding 368 amino acids. The predicted relative molecular mass was 41.05KD and the isoelectric point was 6.03. There is a highly conserved UDPGT structural domain from positions 155 to 336, which is highly homologous to the same species Carnation by multiple sequence alignments. Conclusion The UGT71G1 gene was successfully cloned and analyzed, providing the basis for the study of the key enzyme genes and their regulatory mechanisms.