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应用高效波相色谱法测定了杜仲中京尼平甙的含量。采用YWG-C_(18)柱,甲醇-水-冰醋酸(28:70:2)为流动相,于240nm波长处检测,平均回收率为99.55％,RSD为1.58％。本法简单、准确、重现性好。
The content of genipin in Eucommia ulmoides was determined by high performance wave-phase chromatography. The YWG-C_(18) column with methanol-water-glacial acetic acid (28:70:2) as the mobile phase was detected at 240nm wavelength. The average recovery was 99.55% and the RSD was 1.58%. This method is simple, accurate, and reproducible.