采用花粉管通道法,将野生碱蓬总DNA导入玉米自交系“美系天杂”。对导入后代M-3及受体进行0、150、200、250 mmol/L Na Cl浓度梯度处理,结果表明,各处理浓度导入系根和叶片中SOD含量均高于受体且差异显著,MDA含量比受体低且差异显著,推测导入系耐盐性有所提高。采用SSR标记进一步分析导入后代,引物phi065检测到导入系M-3与供体有相同带型。针对相同带型DNA片段测序分析,证明碱蓬DNA片段可能成功整合到玉米受体“美系天杂”基因组中,但整合碱基序列存在包括碱基的转换、颠换、缺失和插入4种类型变异。 Using pollen tube channel method, the total DNA of wild Suaeda into maize inbred lines The results showed that the concentration of SOD in the roots and leaves of all the treatments was higher than that of the recipient and the difference was significant. MDA Lower than the receptor content and the significant difference, suggesting that the introduction of salt-tolerant system has increased. The SSR marker was used to further analyze and introduce the progeny. The primer phi065 detected that the introduction line M-3 had the same band pattern as the donor. Sequencing and analysis of DNA fragments of the same band proved that the DNA fragment of Suaeda microphylla could be successfully integrated into the genome of maize receptor “MeiXiatianza”, but the integration of the base sequence includes base conversion, transversion, deletion and insertion 4 types of variation.