论文部分内容阅读
目的 观察哮喘患者吸入糖皮质激素 (激素 )前后肺泡巨噬细胞 (AM)释放白细胞介素 - 10 (IL -10 )的能力。方法 对 8例健康对照者和 10例哮喘患者吸入激素治疗前后进行支气管肺泡灌洗 (BAL)获取AM ,经脂多糖 (LPS)刺激培养后取上清液用双抗体夹心酶联免疫吸附法 (ELISA)测量IL - 10含量。结果 哮喘病人吸入治疗前IL - 10水平明显低于健康对照组 ,而治疗后则明显上升。结论 AM释放IL - 10的能力下降是哮喘病人的一个病理特征 ,而激素吸入治疗可提高IL - 10的现象对哮喘治疗具有十分有益的意义
Objective To investigate the ability of alveolar macrophages (AM) to release interleukin - 10 (IL - 10) before and after glucocorticoid (hormone) inhalation in asthmatic patients. Methods AM was obtained by bronchoalveolar lavage (BAL) before and after inhaled steroid treatment in 8 healthy controls and 10 asthmatic patients. After stimulated by lipopolysaccharide (LPS), the supernatants were harvested and analyzed by double antibody sandwich enzyme-linked immunosorbent assay ELISA) to measure IL-10 content. Results The levels of IL - 10 in asthmatic patients before inhalation were significantly lower than those in healthy controls, but obviously increased after treatment. Conclusion The decreased ability of AM to release IL - 10 is a pathological feature of asthmatic patients, and the hormone inhalation therapy can improve the effect of IL - 10 on the treatment of asthma