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目的:建立安神养血口服液的含量测定方法。方法:运用HPLC测定安神养血口服液中钩藤碱和异钩藤碱含量。色谱柱:依利特Kromasil C18柱(5μm,4.6mm×250mm);流动相:甲醇-水(55∶45)(含0.01mol/L三乙胺,用冰醋酸调pH值至7.5);柱温:25℃;检测波长:254nm;流速:1.0mL/min。结果:钩藤碱的平均回收率为99.04%,异钩藤碱为98.75%。钩藤碱在进样量0.12~1.20μg范围内线性关系良好(r1=0.9999),异钩藤碱在进样量0.08~0.80μg范围内线性关系良好(r2=0.9993)。结论:该方法简便、快速、准确,可用于安神养血口服液的质量控制。
Objective: To establish a method for the determination of Anshen Nourishing Oral Liquid. Methods: HPLC was used to determine the content of rhynchophylline and isoflavine in Anshen Yangxue Oral Liquid. Column: Column Kromasil C18 (5μm, 4.6mm × 250mm); mobile phase: methanol-water (55:45) (containing 0.01mol/L triethylamine, adjusted to pH 7.5 with glacial acetic acid); column temperature : 25°C; detection wavelength: 254 nm; flow rate: 1.0 mL/min. Results: The average recovery rate of rhynchophylline was 99.04% and that of isorhynchoine was 98.75%. Rhynchophylline had a good linearity in the range of 0.12~1.20μg injection volume (r1=0.9999), and had a good linear relationship (r2=0.9993) in the range of 0.08~0.80μg injection volume. Conclusion: This method is simple, rapid, accurate, and can be used to control the quality of Anshen Nourishing Oral Liquid.