报道了使用多价血清同时检测多种马铃薯病毒的快速DAS ELISA法。实验分别用快速DAS ELISA法和常规DAS ELISA法检测PVX、PVY、PVS、PVM、PLRV5种主要马铃薯病毒进行比较 ,二者阳性率和灵敏度基本相同 ,但快速法比常规法操作简便、节省时间、节省材料 ,且快速法重复性好 ,结果可靠 ,表明快速DAS ELISA法是一种直观、实用、快速、准确可靠的检测方法 ,适合种薯生产中大量样品的多种主要马铃薯病毒的快速检测。 A rapid DAS ELISA method for the simultaneous detection of various potato viruses using multivalent sera was reported. The rapid detection of PVX, PVY, PVS, PVM and PLRV were carried out by rapid DAS ELISA and conventional DAS ELISA respectively. The positive rate and sensitivity of the two viruses were basically the same. However, the rapid method was simpler than the conventional method, saving time, Saving materials, fast reproducibility and reliable results, indicating that the rapid DAS ELISA method is an intuitive, practical, rapid and accurate detection method suitable for the rapid detection of a large number of major potato viruses in potato production.