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目的通过建立真菌透甲模型,探讨林生地霉、白地霉在体外能否侵犯健康甲板。方法以红色毛癣菌作为阳性对照,制备透甲模型,分为林生地霉组、白地霉组和红色毛癣菌组,观察并记录各组透甲时间,采用统计学方法比较是否存在差异性。采用扫描电镜观察受侵甲板的微观变化。结果三株真菌的菌丝均能穿透甲板,其中林生地霉组的透甲时间为(15.07±3.28)d,白地霉组为(14.50±1.67)d,红色毛癣菌组为(6.76±1.72)d。林生地霉组和白地霉组的透甲时间均明显长于红色毛癣菌组,差异有统计学意义(P<0.05),林生地霉组和白地霉组的透甲时间差异无统计学意义(P>0.05)。扫描电镜观察到三种菌株均有菌丝从角化细胞缝隙及细胞中央穿过,相对于健康甲板,受侵甲板背侧面角化细胞“鱼鳞样”排列消失,边缘模糊,细胞间隙宽窄不等,腹侧面角化细胞未见明显变化。结论林生地霉和白地霉均能穿透甲板,但侵袭力较红色毛癣菌弱。
Objective To establish a model of fungus toxemeta to explore whether Limonium candidum and Geotrichum candidum can invade healthy decks in vitro. Methods Trichophyton rubrum was used as a positive control to make the model of peritoneal cavity. The models were divided into Linthicum, the white mold and the red Trichophyton rubrum. The time of transdermal formation of each group was observed and recorded. The differences were statistically analyzed . Scanning electron microscopy was used to observe the microscopic changes of the invaded deck. Results The mycorrhizal fungi could penetrate the deck of the three strains of fungi. The duration of transdermal larvae was (15.07 ± 3.28) days in A.stredivis, and (14.50 ± 1.67) days in A. germatris and 6.76 ± 1.72) d. The transdermal lavage times of L. linter and C. albicans were significantly longer than that of T. rubrum, the difference was statistically significant (P <0.05) P> 0.05). Scanning electron microscopy showed that all the three strains of mycelium from the keratinocyte gap and the central cell through, relative to the health deck, the dorsal horn keratinocytes “fish scale like ” arrangement disappeared, fuzzy edge, cell gap width Unequal, ventral surface keratinocytes no significant change. Conclusion Both Geotrichum candidum and Geotrichum candidum penetrate the deck, but the invasiveness is weaker than that of Trichophyton rubrum.