利用AFLP标记技术,结合BSA法,用256个EcoRⅠ/MseⅠ(3+3)引物组合对西瓜核雄性不育两用系G17AB进行了分析,得到了一条特异的扩增条带E31M50。用该引物对后代分离群体进行分析,确定了E31M50与育性基因的遗传距离为5.0cM。通过对这个多态性特异片段进行回收和测序,得到了这个337bp片段的全长序列。该标记可以用于分子辅助育种和不育株早期筛选。 By using AFLP marker combined with BSA method, a pair of 256 EcoRⅠ / MseⅠ (3 + 3) primer combinations were used to analyze the G17AB male sterile line of watermelon, and a specific amplification band E31M50 was obtained. The progeny segregation population was analyzed by this primer, and the genetic distance between E31M50 and fertile gene was 5.0 cM. Through the recovery and sequencing of this polymorphism-specific fragment, the full-length sequence of this 337 bp fragment was obtained. The marker can be used for molecular-assisted breeding and early screening of sterile plants.