1983年Hedner和Kisiel采用灭菌技术从人血浆中纯化出因子Ⅶa,之后将纯化的因子Ⅶa用于2例产生高滴度抗体的血友病A的治疗,出血症状很快控制。随后纯化的因子Ⅶa越来越多用于常规治疗无效且已产生抑制物的血友病的出血治疗,均产生很好的疗效。但此种方法提纯因子Ⅶa需要消耗大量 In 1983 Hedner and Kisiel purified Factor Ⅶa from human plasma using a sterilizing technique and then purified Factor Ⅶa for the treatment of 2 patients with high titers of hemophilia A with rapid control of bleeding symptoms. Subsequent purification of Factor VIIa is increasingly used to treat haemorrhages that are ineffective in conventional treatment and have produced inhibitors, and produce good results. However, this method requires a large amount of factor VIIa purification