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目的研究外周血吲哚胺2,3-双加氧酶(IDO)和梅毒血清固定的关系。方法采用前瞻性病例对照研究的方法,用Ficoll-Paque密度梯度离心法分离血清固定患者与无血清固定患者和健康对照组的外周血单个核细胞(PBMC),用酶联免疫吸附试验(ELISA)检测PBMC中的IDO。结果梅毒患者的IDO蛋白要显著高于健康对照,血清固定患者的IDO蛋白要显著高于无血清固定的患者,多变量Logistic回归分析模型显示梅毒患者外周血IDO水平升高与血清固定密切相关。结论 IDO可能参与了梅毒血清固定和免疫耐受。
Objective To study the relationship between peripheral blood indoleamine 2,3-dioxygenase (IDO) and syphilis serum. Methods Using prospective case-control study, peripheral blood mononuclear cells (PBMC) from patients with serum-fixed and non-serum-fixed patients and healthy controls were separated by Ficoll-Paque density gradient centrifugation and detected by enzyme linked immunosorbent assay (ELISA) Detect IDO in PBMCs. Results The IDO protein in syphilis patients was significantly higher than that in healthy controls. IDO protein in serum-fixed patients was significantly higher than that in serum-free patients. Multivariate Logistic regression analysis showed that IDO levels in peripheral blood of syphilis patients were closely related to serum levels of IDO. Conclusion IDO may participate in syphilis serum fixation and immune tolerance.