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目的通过观察子痫前期患者血清中IFN-γ、IL-4水平,来探讨Th1、Th2型细胞因子在子痫前期疾病发病中所起的作用。方法用ELISA法分别测定正常孕妇15例、重度子痫前期患者15例、轻度子痫前期患者15例血清中IFN-γ、IL-4水平及计算IFN-γ/IL-4的比值。结果①血清中IFN-γ:轻度子痫前期组与正常妊娠组差异无统计学意义(P>0.05);重度子痫前期组明显高于正常妊娠组、轻度子痫前期组,差异有统计学意义(P<0.05)。②血清中IL-4:轻度子痫前期组与正常妊娠组相比差异无统计学意义(P>0.05);重度子痫前期组与轻度子痫前期组相比差异无统计学意义(P>0.05);重度子痫前期组低于正常妊娠组,差异有统计学意义(P<0.05)。③血清中IFN-γ/IL-4:轻度子痫前期组与正常妊娠组相比差异无统计学意义(P>0.05);重度子痫前期组与轻度子痫前期组相比差异无统计学意义(P>0.05);重度子痫前期组明显高于正常妊娠组,差异有统计学意义(P<0.05)。结论 IFN-γ和IL-4的表达失衡和紊乱可能是子痫前期免疫学发病机制中的重要环节,Th1/Th2的失衡可能是妊娠期高血压疾病发生机制之一。
Objective To investigate the role of Th1 and Th2 cytokines in the pathogenesis of preeclampsia by observing the levels of IFN-γ and IL-4 in serum of preeclampsia patients. Methods Serum levels of IFN-γ and IL-4 in 15 pregnant women, 15 severe pregnant women with severe preeclampsia and 15 mild mild preeclampsia were measured by ELISA. The ratio of IFN-γ / IL-4 was calculated. Results ① serum IFN-γ: mild preeclampsia group and normal pregnancy group no significant difference (P> 0.05); severe preeclampsia group was significantly higher than normal pregnancy group, mild preeclampsia group, the difference was Statistical significance (P <0.05). ② There was no significant difference in serum IL-4: mild preeclampsia group and normal pregnancy group (P> 0.05); there was no significant difference between severe preeclampsia group and mild preeclampsia group P> 0.05); severe preeclampsia group than in normal pregnancy group, the difference was statistically significant (P <0.05). ③Serum IFN-γ / IL-4: There was no significant difference between mild preeclampsia group and normal pregnancy group (P> 0.05); There was no significant difference between severe preeclampsia group and mild preeclampsia group Statistical significance (P> 0.05); severe preeclampsia group was significantly higher than normal pregnancy group, the difference was statistically significant (P <0.05). Conclusion The imbalance of IFN-γ and IL-4 expression may be an important link in the immunological pathogenesis of preeclampsia. The imbalance of Th1 / Th2 may be one of the mechanisms of hypertensive disorder complicating pregnancy.