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内源性阿片肽在心血管功能调节中的作用,日益受到人们的重视。本实验应用放射免疫测定法(RIA),观察了大鼠左冠状动脉前降支结扎后的不同时间,血浆、垂体、部分脑区及心肌中β-内啡呔与强啡呔A_(1-13)免疫活性物质(ir-β-EP、ir-DynA(1-13))含量的动态变化,与临床急性梗塞的诊治提供实验依据。材料和方法体重200～220g的雄性SD大鼠80只,随机分为对照组与结扎组。尿酯(1.3g/kg)腹腔麻醉后,右股动脉插管,记录血压。气管插管,接呼吸机。于胸骨左缘第3、4肋间开胸,暴露心脏,剪开心包膜,用 5/0号丝线结扎左冠状动脉根部。对照组只穿线而不结扎。在冠状动脉
The role of endogenous opioid peptides in the regulation of cardiovascular function has attracted increasing attention. In this experiment, radioimmunoassay (RIA) was used to observe β-endorphin and dynorphin A_(1-) in plasma, pituitary, partial brain regions, and myocardium at different times after ligation of the left anterior descending coronary artery. 13) Dynamic changes in the content of immunoreactive substances (ir-β-EP, ir-DynA(1-13)) provide experimental evidence for the diagnosis and treatment of clinical acute infarction. Materials and Methods Eighty male Sprague Dawley rats weighing 200-220 g were randomly divided into a control group and a ligation group. After urinary ester (1.3 g/kg) was abdominally anesthetized, the right femoral artery was cannulated and the blood pressure recorded. Intubation, then ventilator. Open the chest between the 3rd and 4th intercostal space on the left sternal border, expose the heart, cut the happy capsule, and ligate the left coronary artery root with 5/0 silk thread. The control group only threaded without ligation. In coronary artery