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目的了解门冬氨酸钾对肝细胞内钾离子浓度及细胞膜Na+·K+-ATP酶活性的影响。方法人和大鼠肝细胞株培养传代,通过CCK-8测细胞活力确定门冬氨酸钾和氯化钾分别作用于两株肝细胞的合适浓度,利用该浓度处理细胞,培养0、24和48h后破碎细胞取上清液测定两株肝细胞内钾离子浓度,提取细胞膜定磷法测定细胞膜Na+·K+-ATP酶活性。统计学处理采用t检验,方差分析及LSD法。结果与空白组和氯化钾组相比,门冬氨酸钾组K+进入细胞内的量明显增多(P<0.05或P<0.01),在24h、48h两个时间点,L02细胞内K+浓度比KCl组分别升高了31%和38%,比空白组分别升高了62%和73%;BRL细胞内K+浓度比KCl组分别升高了21%和40%,空白组分别升高了52%和68%。且其细胞膜Na+·K+-ATP酶活性升高(P<0.01),但两株细胞间均无明显差异。结论门冬氨酸钾能促进K+进入细胞内,并提高了肝细胞膜Na+·K+-ATP酶的活性。
Objective To investigate the effect of potassium aspartate on intracellular potassium concentration and membrane Na + · K + -ATPase activity in hepatocytes. Methods Human and rat hepatocyte cell lines were cultured and passaged. The viability of CCK-8 cell line was measured to determine the appropriate concentration of potassium aspartate and potassium chloride to act on the two hepatocytes respectively. Cells were treated with this concentration and cultured for 0, 24 and After 48h, the supernatant of the supernatant was used to measure the concentration of potassium in the two hepatocytes, and the activity of Na + · K + -ATPase in the cell membrane was determined by determining the content of P in the cell membrane. Statistical analysis using t test, analysis of variance and LSD method. Results Compared with the blank group and the potassium chloride group, the amount of K + entering the cells in the potassium aspartate group increased significantly (P <0.05 or P <0.01). At the two time points of 24h and 48h, K + concentration in L02 cells Which was increased by 31% and 38% respectively compared with KCl group, increased by 62% and 73% respectively compared with blank group; K + concentration in BRL increased by 21% and 40% 52% and 68%. The activity of Na + · K + -ATPase increased (P <0.01), but there was no significant difference between the two cell lines. Conclusion Potassium aspartate can promote the entry of K + into the cells and increase the activity of Na + · K + -ATPase in liver cells.