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目的:研究二苯乙烯苷(2,3,5,4’tetrahydroxystilbene-2-O-β-D-glucoside,THSG)对体外培养的人乳腺癌MCF-7细胞增殖的作用及对PI3K/Akt信号通路的影响。方法:MTT法检测细胞增殖活性,AnnexinⅤ/PI双标法检测细胞凋亡,Western印迹法检测PTEN、p-PKB、cyclinD1、Bcl-2、caspase3和NF-κB蛋白的表达,RT-PCR检测PI3K上游抑制物PTEN mRNA的表达。结果:1、10和100μmol/L的THSG处理细胞24、48和72h后,明显抑制MCF-7细胞增殖,100μmol/L作用48h的增殖抑制率为41.8%(P<0.05),但低浓度(0.1、0.01μmol/L)THSG出现弱的促增殖作用。1、10和100μmol/LTHSG作用48h后,MCF-7细胞凋亡率分别为(6.25±0.65)%、(5.04±0.83)%和(7.98±0.67)%。1、10和100μmol/LTHSG上调PTEN的蛋白表达量,下调p-PKB和cyclin D1的蛋白表达,均呈剂量依赖性;而对NF-κB、Bcl-2和caspase-3表达却无明显影响。THSG能轻微上调PTEN mRNA水平。结论:THSG可抑制MCF-7细胞增殖,该作用可能是通过抑制PI3K/Akt信号通路实现的。
AIM: To investigate the effect of 2,3,5,4’tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on the proliferation of human breast cancer cell line MCF-7 cultured in vitro and its effect on PI3K / Akt signaling The impact of access. Methods: Cell proliferation was detected by MTT assay. Apoptosis was detected by AnnexinⅤ / PI double staining and Western blotting was used to detect the expression of PTEN, p-PKB, cyclinD1, Bcl-2, caspase3 and NF- Upstream Inhibitor PTEN mRNA Expression. Results: The proliferation of MCF-7 cells was inhibited by 1, 10 and 100 μmol / L THSG for 24, 48 and 72 h, respectively. The inhibition rate of proliferation was 41.8% (P <0.05) at 100 μmol / 0.1,0.01μmol / L) THSG appeared a weak role in promoting proliferation. The apoptotic rates of MCF-7 cells treated with 1, 10 and 100 μmol / L of LTHSG for 48 h were (6.25 ± 0.65)%, (5.04 ± 0.83)% and (7.98 ± 0.67)%, respectively. 1, 10 and 100μmol / LTHSG upregulated PTEN protein expression, down-regulated the protein expression of p-PKB and cyclin D1 in a dose-dependent manner, while had no effect on the expression of NF-κB, Bcl-2 and caspase-3. THSG slightly up-regulates PTEN mRNA levels. Conclusion: THSG can inhibit the proliferation of MCF-7 cells, which may be through the inhibition of PI3K / Akt signaling pathway.