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目的评价反向线点杂交方法检测生殖支原体、人型支原体、微小脲原体和解脲脲原体的敏感性。方法应用支原体反向线点杂交方法和种特异性PCR方法同时检测198例临床标本,比较两种方法的检测结果。结果支原体反向线点杂交方法和种特异性PCR检测支原体阳性率分别为33.3%和34.3%,两种方法检测结果符合率为98.5%。3例标本支原体种特异性PCR方法检测微小脲原体阳性,而反向线点杂交方法检测为阴性。两种方法检测支原体结果无显著性差异(P>0.05)。结论反向线点杂交方法能同时快速、敏感和特异的检测这4种支原体。
Objective To evaluate the sensitivity of reverse line hybridization to detect Mycoplasma genitalium, Mycoplasma hominis, Micro-ureas and Ureaplasma urealyticum. Methods The mycoplasma reverse line hybridization method and the species-specific PCR method were used to detect 198 clinical specimens at the same time. The results of two methods were compared. Results The positive rate of Mycoplasma positive for mycoplasma by reverse line hybridization and species-specific PCR was 33.3% and 34.3%, respectively. The coincidence rate of the two methods was 98.5%. Three specimens of mycoplasma species-specific PCR method for detection of M. minituberculosis positive, while the reverse line hybridization method was negative. There was no significant difference between the two methods in detecting mycoplasma (P> 0.05). Conclusion The back line hybridization method can detect these four mycoplasmas quickly, sensitively and specifically.