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目的 :探讨多个相关基因蛋白产物与膀胱癌生物学行为的关系。方法 :采用免疫组化 (SP)法检测 5 8例 (6 3份 )膀胱移行细胞癌 (BTCC)石腊病理切片及 6例正常膀胱组织切片中 P5 3、P1 6 蛋白和增殖细胞核抗原 (PCNA)的表达情况 ,并与病理分级、临床分期及随防结果相比较。结果 :仅一项或无一项指标表达异常的 1 8例 (31 .0 3% ) ,2项或 2项以上指标表达异常的 40例 (6 8.9% )。本组 BTCC大多数表现为多项基因蛋白产物表达异常。两组间比较在病理分级、临床分期上有显著性关系 (P<0 .0 0 5 ) ,并且与肿瘤复发及患者生存情况有显著性关系 (P<0 .0 0 5 )。同时检测多项基因蛋白产物的表达作为估价 BTCC的生物学行为指标 ,可能较单一基因蛋白产物表达更具有意义
Objective: To investigate the relationship between multiple related protein products and biological behavior of bladder cancer. Methods: Immunohistochemistry (SP) was used to detect the expression of P53, P1 6 protein and proliferating cell nuclear antigen (PCNA) in 58 specimens of bladder transitional cell carcinoma (BTCC) and 6 normal bladder tissue sections ) Expression, and with the pathological grade, clinical stage and with the results compared. Results: Eighteen cases (31.0%) with abnormal expression of one or none of the indicators and 40 cases (6.9%) with abnormal expression of two or more indicators. The majority of this group of BTCC showed abnormal expression of multiple gene protein products. There was a significant correlation between the two groups in pathological grade and clinical stage (P <0.05), and there was a significant relationship between tumor recurrence and patient survival (P <0.05). Simultaneous detection of the expression of several gene protein products may be more meaningful than the expression of a single gene protein product as a biological behavioral index for evaluating BTCC