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为了研究炎消热清(AEE)的镇痛作用及其可能的机制,采用热板法和扭体法考察AEE的镇痛效果,并观察热刺激下二乙基二硫代氨基甲酸钠(DDC)、氟哌啶醇(Hal)、L-色氨酸(L-Trp)、赛庚啶(CYP)等药物对AEE镇痛作用的影响,用紫外分光光度法测定了热刺激所致疼痛小鼠全脑中的前列腺素E2(PGE2)含量。结果表明,AEE可明显减少醋酸扭体试验中小鼠的扭体次数,降低热刺激小鼠疼痛反应;可显著降低热刺激所致疼痛小鼠全脑中PGE2的含量和二乙基二硫代氨基甲酸钠对痛觉的敏感度,与氟哌啶醇之间存在明显的协同效应,与L-色氨酸和赛庚啶之间拮抗效应显著。说明AEE具有明显的镇痛作用,镇痛机制可能与其降低小鼠全脑中的PGE2含量以及增加去甲肾上腺素(NE)的含量、阻断多巴胺(DA)受体有关。
In order to study the analgesic effect and its possible mechanism of Atherosclerosis (AEE), the analgesic effect of AEE was investigated by hot plate method and writhing method. The effects of heat-stimulated sodium diethyldithiocarbamate (DDC) , Haloperidol (L-Trp), cyproheptadine (CYP) and other drugs on the analgesic effect of AEE were measured by ultraviolet spectrophotometry of heat-induced pain in mice Prostaglandin E2 (PGE2) content in whole brain. The results showed that AEE could significantly reduce the writhing times of mice in writhing test and reduce the pain response in mice stimulated by heat. It could significantly reduce the content of PGE2 in whole brain of mice with pain induced by heat stimulation and the activity of diethyldithiamino The sensitivity of sodium formate to pain and haloperidol have a significant synergistic effect with L-tryptophan and cyproheptadile significant antagonistic effect. The results showed that AEE had a significant analgesic effect. The analgesic mechanism may be related to the decrease of PGE2 content in the whole brain of mice and the increase of norepinephrine (NE) content and the block of dopamine (DA) receptors.