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目的探讨丙戊酸钠(VPA)对人肝癌Bel-7402细胞增殖抑制和诱导凋亡作用。方法采用四甲基偶氮唑蓝(MTT)比色法检测细胞生长的抑制作用,流式细胞术检测细胞凋亡率,免疫组化和Western blot印迹方法检测血管内皮生长因子(VEGF)蛋白的表达水平。结果 VPA对Bel-7402细胞的生长抑制作用呈现时间和剂量依赖性;经1、2和4 mmol/L VPA作用细胞72 h后,细胞的凋亡率由处理前的(2.78±0.32)%,上升为(8.79±0.53)%、(18.65±1.02)%和(36.41±1.93)%;VEGF蛋白的表达明显呈浓度依赖性降低(P<0.05)。结论 VPA对Bel-7402细胞有显著的增殖抑制及诱导凋亡作用,其作用机制可能与下调VEGF的表达有关。
Objective To investigate the effect of VPA on the proliferation and apoptosis of human hepatoma Bel-7402 cells. Methods MTT assay was used to detect the cell growth inhibition. Flow cytometry was used to detect the apoptosis rate. Immunohistochemistry and Western blotting were used to detect the expression of vascular endothelial growth factor (VEGF) The expression level. Results VPA inhibited the growth of Bel-7402 cells in a time-and dose-dependent manner. The apoptotic rates of VPA-treated cells were significantly decreased from (2.78 ± 0.32)% before treatment to 1, 2 and 4 mmol / L VPA for 72 h, (8.79 ± 0.53)%, (18.65 ± 1.02)% and (36.41 ± 1.93)%, respectively. The expression of VEGF protein was significantly decreased in a concentration-dependent manner (P <0.05). Conclusion VPA can significantly inhibit Bel-7402 cell proliferation and induce apoptosis, and its mechanism may be related to the down-regulation of VEGF expression.