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[目的]从分子水平上了解贵州省甘蓝型油菜主推品种的遗传多样性。[方法]利用国家油菜区域试验DNA指纹鉴定使用的40对SSR引物对贵州省9个甘蓝型油菜主推品种进行多态性检测,并进行聚类分析。[结果]试验共扩增出条带191个,其中多态性条带143个,多态性比率为75%。经聚类分析,9个油菜品种被分为A、B 2簇,其中A簇包含7个品种,B簇为贵州省油料研究所选育的2个品种。A簇在相似系数0.643 4处又分为A1和A2 2个亚簇,A1亚簇为贵州省油菜研究所选育的3个品种,A2亚簇为贵州省油料研究所选育4个品种。[结论]同一单位选育出的品种都有更为接近的遗传背景;在该研究所用品种中,贵州省油料研究所选育的品种遗传基础相对较广,但黔油17与黔油29的遗传相似性系数达0.87,遗传基础较为相近。
[Objective] The research aimed to understand the genetic diversity of the main push varieties of Brassica napus in Guizhou Province at the molecular level. [Method] 40 pairs of SSR primers used for DNA fingerprinting in the national rapeseed test were used to detect the polymorphism of 9 main rape varieties in Guizhou Province and cluster analysis was conducted. [Result] A total of 191 bands were amplified, of which 143 were polymorphic bands with a polymorphic ratio of 75%. According to the cluster analysis, nine rapeseed cultivars were divided into A and B 2 clusters, among which A cluster contained 7 varieties and B cluster was selected by Guizhou Provincial Institute of Oil Crops. Cluster A was divided into A1 and A2 sub-clusters at the similarity coefficient of 0.643 4. Cluster A1 was selected from Guizhou Rapeseed Institute, and cluster A2 was selected from Guizhou Provincial Institute of Oil Crops. [Conclusion] The breeds bred by the same unit had a closer genetic background. Among the cultivars used in this study, the genetic basis of Guizhou breeds was relatively broad, but the relationships between Qianyou 17 and Qianyou 29 Genetic similarity coefficient of 0.87, the genetic basis is similar.