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贵州某金矿含金3.05 g.t-1、砷0.25%、硫5.95%、碳质1.44%,金粒度极小(多数小于2μm),采用浮选工艺获得的金精矿金品位低,硫砷难以分离,金精矿直接氰化浸出金浸出率只有10.21%,为典型的难选冶金矿石。针对浮选金精矿的特点,开展了高效浸矿菌的选育、生物预氧化-氰化浸出实验研究。实验结果表明,采自泥堡金矿(NB)、紫金山铜矿(ZJ)、白山镍钴铜矿(BS)、墨江镍钴矿(MJ)、德兴铜矿(DX)等五个矿山的浸矿菌群中NB菌生长繁殖最好,故选定NB菌群作为实验用菌种,经过进一步的连续转接、逐级驯化后,用于生物预氧化,氧化周期15 d,硫的氧化率达到49.31%,金的氰化浸出率达到80.18%。采用构建16SrDNA克隆文库的方法对预氧化体系中的微生物组成进行了初步研究,结果表明,预氧化体系中NB菌群既包含细菌也包括古菌,细菌主要由Sulfobacillus sp.(52%),Sulfobacillus thermotoler-ans(25%),Leptospirillum ferriphilum(23%)组成,而古菌为Ferroplasma acidiphilum。
The gold content of a gold mine in Guizhou Province is 3.05 gt-1, 0.25% arsenic, 5.95% arsenic and 1.44% carbonaceous. The gold particle size is very small (mostly less than 2μm) Separation, direct gold cyanide leaching gold leaching rate of only 10.21%, a typical refractory metallurgical ore. According to the characteristics of flotation gold concentrate, the breeding of high-efficiency leaching bacteria and biological pre-oxidation-cyanidation leaching experiments were carried out. The experimental results show that the five mines collected from Nibao, Zijishan, BS, MJ and DX Of the bacteria in the leaching flora of the NB growth and reproduction of the best, so choose NB bacteria as experimental strains, after further continuous transfer, gradual domestication, for biological pre-oxidation, oxidation cycle 15 d, sulfur The oxidation rate reached 49.31%, gold cyanide leaching rate reached 80.18%. Microbial composition in the pre-oxidation system was preliminary studied by constructing a 16S rDNA clone library. The results showed that the bacteria in the pre-oxidation system contained both bacteria and archaea. The bacteria mainly consisted of Sulfobacillus sp. (52%), Sulfobacillus thermotoler-ans (25%), Leptospirillum ferriphilum (23%), and archaeal Ferroplasma acidiphilum.