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苎麻疫霉(Phytophthora boehmeriae Sawada.)菌株在利马豆培养基平板上形成的卵孢子,用KMnO_4和 H2O_2处理,在 S+L 培养基上诱导萌发(25℃).结果表明,用 KMnO_4和 H_2O_2处理卵孢子均能刺激其萌发.在一定时期内卵孢子萌发率随卵孢子保存时间的增加而上升,保存35d 的卵孢子用0.3%H_2O_2处理2min 和保存45d 的卵孢子用0.1%KMnO_4处理20min,萌发率最高,分别为80%和92%.卵孢子萌发时给予光照对萌发有明显刺激作用,卵孢子在萌发过程中的光照条件以黑光灯连续照射效果最好,日光灯连续照射效果较差.所测定的4种培养基中以 S+L 对卵孢子的萌发效果最好,其次为SA 和 WA.
The oospores formed on the plate of Lima bean medium by Phytophthora boehmeriae Sawada strain were treated with KMnO 4 and H 2 O 2 to induce germination (25 ℃) on S + L medium.The results showed that with KMnO 4 and H 2 O 2 The spore germination rate increased with the storage time of oospores in a certain period, and the oospores stored for 35 days were treated with 0.3% H 2 O 2 for 2 min and oospores preserved for 45 days with 0.1% KMnO 4 for 20 min , The highest germination rate was 80% and 92%, respectively.Overosporium germination had obvious stimulating effect on the germination, the illumination condition of the oospores during the germination process was the best when the black light was continuous, and the continuous irradiation was not effective Among the four tested media, S + L had the best germination on oospores, followed by SA and WA.