论文部分内容阅读
目的 :探讨重组腺病毒介导的 IL- 2基因转染的瘤苗的体内抗肿瘤作用及其免疫学机制。方法 :应用腺病毒介导的鼠 IL- 2基因转染 CT2 6小鼠结肠癌细胞 ,灭活后用作瘤苗治疗荷瘤小鼠 ,观察皮下肿瘤生长及其存活期。采用乳酸脱氢酶释放法检测荷瘤小鼠脾细胞 CTL、L AK、NK细胞的杀伤活性。结果 :鼠 IL- 2基因转染瘤苗治疗能显著抑制荷瘤小鼠皮下肿瘤生长并明显延长其存活期 (P<0 .0 1)。体内免疫功能检测表明 ,鼠 IL- 2基因转染疫苗治疗组小鼠脾细胞 CTL 活性、L AK活性和 NK活性显著高于对照组 (P<0 .0 1)。结论 :腺病毒介导鼠 IL- 2基因转染的瘤苗体内具有较强的抗肿瘤效应 ,其机制可能是提高了荷瘤小鼠特异性和非特异性抗肿瘤免疫反应
Objective: To investigate the anti-tumor effect of recombinant adenovirus-mediated IL-2 gene in vitro and its immunological mechanism. Methods: CT2 6 mouse colon cancer cells were transfected with adenovirus mediated murine IL-2 gene and used as tumor vaccine in tumor-bearing mice after inactivation. The growth and survival of subcutaneous tumor were observed. The killing activity of CTL, L AK, NK cells in spleen cells of tumor-bearing mice was detected by lactate dehydrogenase release assay. Results: The transfection of murine IL-2 gene vaccine could significantly inhibit the growth of subcutaneous tumor and prolong the survival of tumor-bearing mice (P <0.01). In vivo immune function tests showed that CTL activity, L AK activity and NK activity of spleen cells in mice treated with murine IL-2 gene vaccine were significantly higher than those in control group (P <0.01). Conclusion: Adenovirus-mediated murine IL-2 gene-transfection vaccine in vivo has a strong anti-tumor effect, the mechanism may be increased tumor-bearing mice specific and non-specific anti-tumor immune response