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目的:探索溴氰菊酯慢性中毒对大鼠胃黏膜的病理损伤及损伤后P物质表达的影响。方法:实验组大鼠分别给予不同剂量溴氰菊酯灌胃,对照组仅给予橄榄油。取胃体部组织,制成石蜡切片,HE及免疫组织化学染色,镜下观察胃黏膜损伤及胃黏膜组织P物质的表达情况,并对损伤程度进行累计积分、壁细胞计数。结果:高剂量组大鼠胃粘膜慢性损害病理积分与各组相比差异均有显著性(P<0.05);壁细胞计数与对照组相比较其差异有P<0.05)。免疫组化发现SP在实验组大鼠胃黏膜组织中呈强阳性表达,在对照组则呈弱阳性表达;平均光密度(OD)值显示高、中剂量组之间及与其余各组之间差异均有显著(P<0.05)。结论:溴氰菊酯对大鼠胃黏膜有较明显的慢性损伤,SP可能参与胃黏膜局部病变。
Objective: To explore the effects of chronic poisoning of deltamethrin on pathological changes of gastric mucosa in rats and the expression of substance P after injury. Methods: The experimental rats were given different doses of deltamethrin orally, the control group was given only olive oil. The body tissues of the stomach were taken and made into paraffin sections, HE and immunohistochemical staining, gastric mucosal damage and the expression of substance P in gastric mucosa were observed microscopically, and the cumulative score and wall cells were counted. Results: The histopathological scores of gastric mucosal chronic injury in high dose group were significantly different from those in each group (P <0.05). The difference of wall cell count between the two groups was significant (P <0.05). The results of immunohistochemistry showed that SP was strongly positive in gastric mucosa of experimental group and weakly positive in control group. The average optical density (OD) value of SP showed that there was significant difference between high and middle dose groups and the other groups The difference was significant (P <0.05). Conclusion: Deltamethrin has obvious chronic injury to gastric mucosa in rats. SP may be involved in the local lesion of gastric mucosa.