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目的:探讨蛋白激酶C抑制剂星形孢菌素(ST)对前列腺癌细胞株PC-3增殖及凋亡的影响。方法:以ST(10-8mol/L)处理体外培养的PC-3细胞株。采用Western印迹检测细胞周期蛋白cyclin A、cyclin D1表达的变化。通过MTT实验、平板克隆实验检测ST对PC-3细胞增殖能力的影响。流式细胞仪检测各组细胞凋亡的情况。光镜观察细胞形态学的改变。结果:PC-3细胞经ST处理后cyclin A、cyclin D1蛋白表达明显降低。MTT实验结果显示ST对PC-3细胞的抑制作用自48 h后(19.35%)有显著性(F=31.06,P<0.01)。平板克隆实验结果显示组细胞克隆形成率ST组[(37.10±3.43)%]明显低于对照组((64.80±4.34)%,χ2=14.59,P<0.05]及DMSO组[(62.80±4.36)%,χ2=12.50,P<0.05]。流式细胞术结果显示ST组凋亡率(19.6±2.20)%与对照组(5.33±1.40)%和DMSO组(5.50±0.96)%比较显著增加(F=104.36,P<0.01)。光镜下可见:与对照组及DMSO组比较ST组细胞生长状态明显变差,细胞变圆,边缘模糊。结论:ST可以抑制前列腺癌细胞的生长和增殖,诱导细胞凋亡。
Objective: To investigate the effect of staurosporine (ST), a protein kinase C inhibitor, on the proliferation and apoptosis of prostate cancer cell line PC-3. Methods: PC-3 cells cultured in vitro were treated with ST (10-8 mol / L). Western blotting was used to detect the changes of cyclin A and cyclin D1 expression. The effects of ST on the proliferation of PC-3 cells were detected by MTT assay and plate clone assay. Flow cytometry was used to detect apoptosis in each group. Light microscopy changes in cell morphology. Results: The expression of cyclin A and cyclin D1 in PC-3 cells was significantly decreased after ST treatment. The results of MTT showed that the inhibitory effect of ST on PC-3 cells was significant after 48 h (19.35%) (F = 31.06, P <0.01). The results of plate clone assay showed that the rate of colony formation in group ST was significantly lower than that in control group [(37.10 ± 3.43)%] (64.80 ± 4.34%, χ2 = 14.59, P <0.05] and [62.80 ± 4.36 %, χ2 = 12.50, P <0.05] .Flow cytometry showed that the apoptotic rate in ST group was significantly higher than that in control group (5.33 ± 1.40)% and DMSO group (5.50 ± 0.96)% F = 104.36, P <0.01) .Conclusion: ST can inhibit the growth and proliferation of prostate cancer cells in ST group compared with control group and DMSO group, Induces apoptosis.